Working with OLIGEN: oleocanthal

Making a Stock Solution of Oleocanthal (molecular weight 304 g/mol)

*Oleocanthal is susceptible to degradation when exposed to light, air or water. Efforts to avoid these conditions as much as possible will extend the life of the compound.
  1. Dissolve 20 mg in 0.5 ml acetone by swirling.
  2. Aliquot 50 μl into ten polypropylene tubes and dry in a vacuum oven keeping the temperature below 65 C.
  3. Flush with Nitrogen and tightly cap.
  4. Store at -20 C. Under these conditions, the compound is stable for at least one year.
  5. The following is a suggestion only. You may prefer to make stock solutions in a different manner. Before starting an experiment, add 131 μl of DMSO to one of the stock vials which will make a 50 mM stock solution. Aliquot 13 μl of this stock solution into 10 microfuge tubes for a working stock. Each tube will be diluted for one experiment to concentrations ranging from 1 μM to 250 μM. We suggest not freeze thawing for reuse. This DMSO working stock is stable for one month stored at -20 C.
  6. 100 μM experimental concentrations will only be 0.2% DMSO but best to have a vehicle control (DMSO at the same concentration as the oleocanthal samples).
Contact Kiley Grant with any Further Questions: Kiley@oleo.live